In the process, the cofactor shuttles between PLP and the pyridoxamine phosphate ( PMP ) form.
2.
In these reactions, the PLP reacts with glutamate, which transfers its alpha-amino group to PLP to make pyridoxamine phosphate ( PMP ).
3.
Water attacks the alpha carbon of the imine of PLP-TYR and through acyl substitution kicks off the nitrogen of PLP and forming pyridoxamine phosphate ( PMP ) and 4-hydroxyphenylpyruvate.
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